4 × 44 k whole human genome microarray slides (Agilent technologies)
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Agilent technologies
4 × 44 k whole human genome microarray slides
4 × 44 K Whole Human Genome Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+human+genome+microarray+(4+%C3%97+44+k)+slide/pmc03610438-112-14-21
Average 90 stars, based on 1 article reviews
4 × 44 K Whole Human Genome Microarray Slides, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/whole+human+genome+microarray+(4+%C3%97+44+k)+slide/pmc03610438-112-14-21
Average 90 stars, based on 1 article reviews
4 × 44 k whole human genome microarray slides - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells"
Article Title: Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells
Journal: Drug Design, Development and Therapy
doi: 10.2147/DDDT.S42390
Figure Legend Snippet: Scatter plot of the gene differential expression in PC-2 cells induced by RPM. Notes: The Cy3 (RPM treated group) and Cy5 (untreated control) channel intensities from the two-color DNA microarray experiments were shown in the scatter plot. The variables appear in a linear relationship, and the linear correlation between them is 0.935. The red plots represented upregulated genes, and the green plots represented downregulated genes. Abbreviation: RPM, rapamycin.
Techniques Used: Expressing, Microarray
Figure Legend Snippet: ( A ) RT-PCR analysis for the DNA-damage repair and transcription genes after the treatment with RPM; ( B ) Difference between the cDNA microarray and RT-PCR in DNA-damage repair and transcription genes. Notes: The genes examined here are: (1) DDB1(NM_001923); (2) RAD51 (NM_002876); (3) XRCC5 (NM_021141); (4) PCNA (NM_002592); and (5) ABCC4 (NM_005845). The values represent the mean ± SD of the data from three independent experiments. Abbreviations: RPM, rapamycin; RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Microarray, Standard Deviation
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Hybridization:Article Title: Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells Article Snippet: The extraction was repeated independently in triplicate. cRNAs labeled with Cyanine 3 were synthesized via the T7-linear amplification method from 500 ng of total RNA using the Low RNA Input Linear Amplification Kit (Agilent Technologies, Palo Alto, CA, USA), and subsequently purified by using the RNeasy Mini Kit (QIAGEN). .. For each hybridization, 1.65 μg of cRNAs was fragmented at 60 °C for 30 min using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized at 65 °C for 17 h to a 4 × 44 K Gene Expression:Article Title: Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells Article Snippet: The extraction was repeated independently in triplicate. cRNAs labeled with Cyanine 3 were synthesized via the T7-linear amplification method from 500 ng of total RNA using the Low RNA Input Linear Amplification Kit (Agilent Technologies, Palo Alto, CA, USA), and subsequently purified by using the RNeasy Mini Kit (QIAGEN). .. For each hybridization, 1.65 μg of cRNAs was fragmented at 60 °C for 30 min using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized at 65 °C for 17 h to a 4 × 44 K Microarray:Article Title: Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells Article Snippet: The extraction was repeated independently in triplicate. cRNAs labeled with Cyanine 3 were synthesized via the T7-linear amplification method from 500 ng of total RNA using the Low RNA Input Linear Amplification Kit (Agilent Technologies, Palo Alto, CA, USA), and subsequently purified by using the RNeasy Mini Kit (QIAGEN). .. For each hybridization, 1.65 μg of cRNAs was fragmented at 60 °C for 30 min using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized at 65 °C for 17 h to a 4 × 44 K Article Title: Differential effects of Paclitaxel on dendritic cell function Article Snippet: Total RNA was extracted from day 7 DC which had been treated for 2 h with 1 μg/ml LPS or 100 μM paclitaxel for 2 h then washed and returned to culture for 24 h. This was used as a template to generate Cy3-labelled cRNA, using the Low RNA Input Linear Amplification Kit (Agilent). .. This was used as a probe on the Article Title: Epithelial TRPV1 Signaling Accelerates Gingival Epithelial Cell Proliferation Article Snippet: Briefly, the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized for 5 min, incubated with TdT end-labeling cocktail for 60 min, and then incubated with anti-fluorescein isothiocyanate (FITC) conjugate for 30 min. To quantitate apoptotic cell death, we calculated the percentage of TUNEL-positive cells relative to total cells after counting the numbers of TUNEL-positive cells and total cells in three random fields using a fluorescent microscope. cDNA Microarray Analysis RNA samples were amplified and labeled with Cy3 and a Quick Amp Labeling Kit (Agilent Technologies, Inc., Santa Clara, CA, USA), according to the manufacturer’s protocol. .. Following labeling and purification, Cy3-labeled cRNAs were competitively hybridized onto an Agilent 4 × 44 K Labeling:Article Title: Epithelial TRPV1 Signaling Accelerates Gingival Epithelial Cell Proliferation Article Snippet: Briefly, the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized for 5 min, incubated with TdT end-labeling cocktail for 60 min, and then incubated with anti-fluorescein isothiocyanate (FITC) conjugate for 30 min. To quantitate apoptotic cell death, we calculated the percentage of TUNEL-positive cells relative to total cells after counting the numbers of TUNEL-positive cells and total cells in three random fields using a fluorescent microscope. cDNA Microarray Analysis RNA samples were amplified and labeled with Cy3 and a Quick Amp Labeling Kit (Agilent Technologies, Inc., Santa Clara, CA, USA), according to the manufacturer’s protocol. .. Following labeling and purification, Cy3-labeled cRNAs were competitively hybridized onto an Agilent 4 × 44 K Purification:Article Title: Epithelial TRPV1 Signaling Accelerates Gingival Epithelial Cell Proliferation Article Snippet: Briefly, the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized for 5 min, incubated with TdT end-labeling cocktail for 60 min, and then incubated with anti-fluorescein isothiocyanate (FITC) conjugate for 30 min. To quantitate apoptotic cell death, we calculated the percentage of TUNEL-positive cells relative to total cells after counting the numbers of TUNEL-positive cells and total cells in three random fields using a fluorescent microscope. cDNA Microarray Analysis RNA samples were amplified and labeled with Cy3 and a Quick Amp Labeling Kit (Agilent Technologies, Inc., Santa Clara, CA, USA), according to the manufacturer’s protocol. .. Following labeling and purification, Cy3-labeled cRNAs were competitively hybridized onto an Agilent 4 × 44 K |
